Journal: International Journal of Molecular Sciences

Article Title: Minimally Manipulative Method for the Expansion of Human Bone Marrow Mesenchymal Stem Cells to Treat Osseous Defects

doi: 10.3390/ijms20030612

Figure Lengend Snippet: Percentage of cell markers expression in cultured hBM-MSC.

Article Snippet: Osteoblasts (HOB) (PromoCell, catalogue C-12720) were grown in Osteoblast Basal Medium (PromoCell, #C27001). hBM-MSC were plated in RPMI-1640 and later on grown in DMEM-high glucose with Pyruvate (Thermo Fisher Scientific) supplemented with 10% patient-derived serum (PDS) and 2% penicillin/streptomycin solution.

Techniques: Expressing, Cell Culture

Journal: International Journal of Molecular Sciences

Article Title: Minimally Manipulative Method for the Expansion of Human Bone Marrow Mesenchymal Stem Cells to Treat Osseous Defects

doi: 10.3390/ijms20030612

Figure Lengend Snippet: Inverted light microscope images of human bone marrow mesenchymal stem cells (hBM-MSC). ( a ) hBM-MSCs cultured from subject BM004 that were grown in 2D culture at 1 week post-harvest (100× magnification); ( b ) hBM-MSCs cultured from subject BM004 that were grown in 2D culture at 3 weeks post-harvest (100× magnification).

Article Snippet: Osteoblasts (HOB) (PromoCell, catalogue C-12720) were grown in Osteoblast Basal Medium (PromoCell, #C27001). hBM-MSC were plated in RPMI-1640 and later on grown in DMEM-high glucose with Pyruvate (Thermo Fisher Scientific) supplemented with 10% patient-derived serum (PDS) and 2% penicillin/streptomycin solution.

Techniques: Light Microscopy, Cell Culture

Journal: International Journal of Molecular Sciences

Article Title: Minimally Manipulative Method for the Expansion of Human Bone Marrow Mesenchymal Stem Cells to Treat Osseous Defects

doi: 10.3390/ijms20030612

Figure Lengend Snippet: Comparison of hBM-MSCs growth using media supplemented with patient-derived serum (PDS) vs. fetal bovine serum (FBS). The diagram represents grow curves (cell number over time) of hBM-MSC (BM001, BM002, and BM004) grown using medium supplemented with FBS Compared to PDS over 72 h.

Article Snippet: Osteoblasts (HOB) (PromoCell, catalogue C-12720) were grown in Osteoblast Basal Medium (PromoCell, #C27001). hBM-MSC were plated in RPMI-1640 and later on grown in DMEM-high glucose with Pyruvate (Thermo Fisher Scientific) supplemented with 10% patient-derived serum (PDS) and 2% penicillin/streptomycin solution.

Techniques: Derivative Assay

Journal: International Journal of Molecular Sciences

Article Title: Minimally Manipulative Method for the Expansion of Human Bone Marrow Mesenchymal Stem Cells to Treat Osseous Defects

doi: 10.3390/ijms20030612

Figure Lengend Snippet: Expression of differentiation markers in hBM-MSC following dexamethasone treatment as measured by Flow cytometer. The histogram is a representative example of the percentages of expression of various bone marrow stem cell and osteoblast differentiation markers, before and after treatment with dexamethasone. Control Osteoblasts were used as positive control. Control osteoblast and BM001 isolated hBM-MSC cells were incubated with anti ALP, CD105, CD44, CD90, Osteopontin, Osteonectin, Osteocalcin, and RUNX2 specific fluoresceinated antibodies and flow cytometry analysis was used to determine percent of expression of the different proteins before and after incubation with dexamethasone to study their differentiation capabilities.

Article Snippet: Osteoblasts (HOB) (PromoCell, catalogue C-12720) were grown in Osteoblast Basal Medium (PromoCell, #C27001). hBM-MSC were plated in RPMI-1640 and later on grown in DMEM-high glucose with Pyruvate (Thermo Fisher Scientific) supplemented with 10% patient-derived serum (PDS) and 2% penicillin/streptomycin solution.

Techniques: Expressing, Flow Cytometry, Positive Control, Isolation, Incubation

Journal: International Journal of Molecular Sciences

Article Title: Minimally Manipulative Method for the Expansion of Human Bone Marrow Mesenchymal Stem Cells to Treat Osseous Defects

doi: 10.3390/ijms20030612

Figure Lengend Snippet: Microscope image of Haematoxylin and Eosin stained scaffolds following culture with hBM-MSCs. ( a ) Representative microscope image of a cross-section of hBM-MSCs seeded and cultured on a 60/40 hydroxyapatite scaffold, and stained with Haematoxylin and Eosin (100× magnification). The viable hBM-MSC population can be seen in significant numbers throughout a full thickness section of the scaffold and the cells do not display prominent apoptotic regions.; ( b ) Representative microscope image of a cross-section of hBM-MSCs seeded and cultured on a PLGA scaffold with 200 µm pores, stained with Haematoxylin and Eosin (100× magnification). The PLGA scaffold did not allow for significant hBM-MSC integration. The MSCs adhered to the outer surfaces of the scaffold and did not migrate far into its interior regions; ( c ) Representative microscope image of a cross-section of hBM-MSCs seeded and cultured on woven chitin fiber material stained with Haematoxylin and Eosin (100× magnification). Also the woven chitin fiber did not support cell growth to a significant degree, showing a viable single cell layer of hBM-MSC cells on the apical and basal surface; ( d ) Representative microscope image of a cross-section of hBM-MSCs seeded and cultured on Allowash cancellous bone scaffold stained with Haematoxylin and Eosin (100× magnification). The Allowash cancellous bone scaffold demonstrated excellent cell integration and promoted MSC proliferation.

Article Snippet: Osteoblasts (HOB) (PromoCell, catalogue C-12720) were grown in Osteoblast Basal Medium (PromoCell, #C27001). hBM-MSC were plated in RPMI-1640 and later on grown in DMEM-high glucose with Pyruvate (Thermo Fisher Scientific) supplemented with 10% patient-derived serum (PDS) and 2% penicillin/streptomycin solution.

Techniques: Microscopy, Staining, Cell Culture

Journal: Scientific Reports

Article Title: Positive impact of Platelet-rich plasma and Platelet-rich fibrin on viability, migration and proliferation of osteoblasts and fibroblasts treated with zoledronic acid

doi: 10.1038/s41598-019-43798-z

Figure Lengend Snippet: Preinvestigation of PRP and PRF for in-vitro addition. The effect of PRP and PRF were examined in different concentrations to fibroblasts ( A ) and osteoblasts ( B ) by real- time cell analysis.

Article Snippet: The fibroblast and osteoblast growth medium (FGM and OGM; both from Provitro®; Berlin Germany) were each supplemented with 10% fetal bovine serum (FBS, Provitro® GmbH) and 1% antibiotics (100 U/ml penicillin G, 100 μg/ml streptomycin).

Techniques: In Vitro, Cell Analysis

Journal: Scientific Reports

Article Title: Positive impact of Platelet-rich plasma and Platelet-rich fibrin on viability, migration and proliferation of osteoblasts and fibroblasts treated with zoledronic acid

doi: 10.1038/s41598-019-43798-z

Figure Lengend Snippet: Representative photographs of cell migration using the scratch assay. The effect of ZA, PRP, PRF and their combination on the migration of fibroblasts ( A ) and osteoblasts ( B ) were examined (PC = positive control; NC = negative control; ZA = zoledronic acid).

Article Snippet: The fibroblast and osteoblast growth medium (FGM and OGM; both from Provitro®; Berlin Germany) were each supplemented with 10% fetal bovine serum (FBS, Provitro® GmbH) and 1% antibiotics (100 U/ml penicillin G, 100 μg/ml streptomycin).

Techniques: Migration, Wound Healing Assay, Positive Control, Negative Control

Journal: Scientific Reports

Article Title: Positive impact of Platelet-rich plasma and Platelet-rich fibrin on viability, migration and proliferation of osteoblasts and fibroblasts treated with zoledronic acid

doi: 10.1038/s41598-019-43798-z

Figure Lengend Snippet: Percentage of closure of the scratch area in fibroblasts ( A ) and osteoblasts ( B ) in the different groups (1.0 = 100% coverage). Significant difference ( p < 0.05) is marked with *.

Article Snippet: The fibroblast and osteoblast growth medium (FGM and OGM; both from Provitro®; Berlin Germany) were each supplemented with 10% fetal bovine serum (FBS, Provitro® GmbH) and 1% antibiotics (100 U/ml penicillin G, 100 μg/ml streptomycin).

Techniques:

Journal: Scientific Reports

Article Title: Positive impact of Platelet-rich plasma and Platelet-rich fibrin on viability, migration and proliferation of osteoblasts and fibroblasts treated with zoledronic acid

doi: 10.1038/s41598-019-43798-z

Figure Lengend Snippet: Effects of PRP, PRF, ZA and their combinations on the viability of human gingival fibroblasts ( A ) and osteoblasts ( B ) assessed by MTT assay. Significant difference ( p < 0.05) is marked with *.

Article Snippet: The fibroblast and osteoblast growth medium (FGM and OGM; both from Provitro®; Berlin Germany) were each supplemented with 10% fetal bovine serum (FBS, Provitro® GmbH) and 1% antibiotics (100 U/ml penicillin G, 100 μg/ml streptomycin).

Techniques: MTT Assay

Journal: Scientific Reports

Article Title: Positive impact of Platelet-rich plasma and Platelet-rich fibrin on viability, migration and proliferation of osteoblasts and fibroblasts treated with zoledronic acid

doi: 10.1038/s41598-019-43798-z

Figure Lengend Snippet: Cell proliferation assessed by real-time cell analysis for fibroblasts ( A ) and osteoblasts ( B ) in the different groups over a period of 72 h. The values are the median of the measured cell index. Significant difference ( p < 0.05) is marked with *.

Article Snippet: The fibroblast and osteoblast growth medium (FGM and OGM; both from Provitro®; Berlin Germany) were each supplemented with 10% fetal bovine serum (FBS, Provitro® GmbH) and 1% antibiotics (100 U/ml penicillin G, 100 μg/ml streptomycin).

Techniques: Cell Analysis